Recombinant human ARFL3/ARL3 protein

ARL3, also known as ARFL3, is a member of the ARF-like protein (ARL) subfamily of small GTPases. unlike ARFs, ARL3 does not activate the cholera toxin ADP-ribosyltranferase. This protein is ubiquitously expressed. It may play a role in maintaining rod photoreceptor cells and regulating microtubule-dependent processes. Knockdown of ARL3 leads to altered cell morphology, failure of cytokinesis, acetylation of alpha tubulin and an increase in binucleated cells. Recombinant human ARL3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

ADP-ribosylation factor-like protein 3, ARFL3, ADP ribosylation factor like GTPase 3

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

23.0 kDa (206aa) confirmed by MALDI-TOF

Additionnal Information

ARL3, ADP-ribosylation factor-like protein 3, ARFL3, ATGP1222-10 µg, ATGP1222-20 µg, ATGP1222-50 µg, ATGP1222-100 µg, ATGP1222-250 µg, ATGP1222-500 µg, ATGP1222-1 mg, ATGP1222-10, ATGP1222-20, ATGP1222-50, ATGP1222-100, ATGP1222-250, ATGP1222-500, ATGP1222-1

References & Citations

Bartolini F. et al. (2002) J Biol Chem. 277:14629-14634.; ; Hillig R.C. et al. (2001) Structure. 8:1239-1245.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 30% glycerol, 0.1M NaCl,1mM DTT, 0.1mM PMSF

Scientific Category

Cancer

NCBI Accession Number

NP_004302

Uniprot Accession Number

P36405

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSHMGLLSI LRKLKSAPDQ EVRILLLGLD NAGKTTLLKQ LASEDISHIT PTQGFNIKSV QSQGFKLNVW DIGGQRKIRP YWKNYFENTD ILIYVIDSAD RKRFEETGQE LAELLEEEKL SCVPVLIFAN KQDLLTAAPA SEIAEGLNLH TIRDRVWQIQ SCSALTGEGV QDGMNWVCKN VNAKKK