Recombinant human SUFU protein

SuFu (Suppressor of fused homolog) belongs to the SuFu family. This protein is negative regulator of the hedgehog signaling pathway. Defects in this gene are a cause of medulloblastoma. SuFu has been shown to interact with GLI1, GLI3 and PEX26. Recombinant human SuFu protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Suppressor of fused homolog, PRO1280, SuFuH, SuFuXL

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

56.1 kDa (504aa)

Additionnal Information

SUFU, suppressor of fused homolog, PRO1280, SuFuH, SuFuXL, ATGP1221-10 µg, ATGP1221-20 µg, ATGP1221-50 µg, ATGP1221-100 µg, ATGP1221-250 µg, ATGP1221-500 µg, ATGP1221-1 mg, ATGP1221-10, ATGP1221-20, ATGP1221-50, ATGP1221-100, ATGP1221-250, ATGP1221-500, ATGP1221-1

References & Citations

Dunaeva M, et al. (2003) J. Biol. Chem. 278 (7) : 5116-22.; ; Murone M., et al. (2000) Nat. Cell Biol. 2:310-312

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Cancer

NCBI Accession Number

NP_057253

Uniprot Accession Number

Q9UMX1

Species

Human

AA Sequence

MAELRPSGAP GPTAPPAPGP TAPPAFASLF PPGLHAIYGE CRRLYPDQPN PLQVTAIVKY WLGGPDPLDY VSMYRNVGSP SANIPEHWHY ISFGLSDLYG DNRVHEFTGT DGPSGFGFEL TFRLKRETGE SAPPTWPAEL MQGLARYVFQ SENTFCSGDH VSWHSPLDNS ESRIQHMLLT EDPQMQPVQT PFGVVTFLQI VGVCTEELHS AQQWNGQGIL ELLRTVPIAG GPWLITDMRR GETIFEIDPH LQERVDKGIE TDGSNLSGVS AKCAWDDLSR PPEDDEDSRS ICIGTQPRRL SGKDTEQIRE TLRRGLEINS KPVLPPINPQ RQNGLAHDRA PSRKDSLESD SSTAIIPHEL IRTRQLESVH LKFNQESGAL IPLCLRGRLL HGRHFTYKSI TGDMAITFVS TGVEGAFATE EHPYAAHGPW LQILLTEEFV EKMLEDLEDL TSPEEFKLPK EYSWPEKKLK VSILPDVVFD SPLH