Recombinant human Ephrin-B2 protein
Ephrin-B2 is a member of the ephrin (EPH) family. The ephrins and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. It binds to the EPHB4 and EPHA3 receptors. Recombinant human EFNB2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
Product Specifications
Product Name Alternative
Ephrin B2, EFNB2, EPH-related receptor tyrosine kinase ligand 5, ligand of eph-related kinase 5, LERK-5, HTK ligand, HTK-L, EPLG5, HTKL
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
24.9 kDa (227aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Jensen, P.L. et al. (2000) Stem Cells 18: 63-6; ; Cerretti, et al. (1995) . Mol. Immunol. (ENGLAND) 32 (16) : 1197-205.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 40% glycerol, 200 mM NaCl, 1mM EDTA
Scientific Category
Neuroscience
NCBI Accession Number
NP_004084.1
Uniprot Accession Number
P52799
Species
Human
AA Sequence
Frequently Asked Questions
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