Recombinant human ARF7/ARL14 protein

ARL14 (ADP-ribosylation factor-like 14) is a developmentally regulated member of the ADP-ribosylation factor/ARF-like protein (ARF/ARL) family of Ras-related GTPases. MHC class II molecules (MHC-II) present peptides to T helper cells to facilitate immune responses and are strongly linked to autoimmune diseases. MHC-II transport was controlled by the GTPase ARL14/ARF7, which recruits the motor myosin 1E via an effector protein ARF7EP. Recombinant human ARL14 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

ADP-ribosylation factor-like protein 14, ARF7, ADP ribosylation factor like GTPase 14, ADP-ribosylation factor 7

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

23.7 kDa (212aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

ARL14, ADP-ribosylation factor-like protein 14, ARF7, ATGP1219-10 µg, ATGP1219-20 µg, ATGP1219-50 µg, ATGP1219-100 µg, ATGP1219-250 µg, ATGP1219-500 µg, ATGP1219-1 mg, ATGP1219-10, ATGP1219-20, ATGP1219-50, ATGP1219-100, ATGP1219-250, ATGP1219-500, ATGP1219-1

References & Citations

Paul P, et al. (2011) Cell. 145 (2) :268-83.; ; Imami K., et al. (2008) Anal. Sci. 24:161-166

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 40% glycerol, 200 mM NaCl, 1mM EDTA

Scientific Category

Signal Transduction

NCBI Accession Number

NP_079323

Uniprot Accession Number

Q8N4G2

Species

Human

AA Sequence

MGSLGSKNPQ TKQAQVLLLG LDSAGKSTLL YKLKLAKDIT TIPTIGFNVE MIELERNLSL TVWDVGGQEK MRTVWGCYCE NTDGLVYVVD STDKQRLEES QRQFEHILKN EHIKNVPVVL LANKQDMPGA LTAEDITRMF KVKKLCSDRN WYVQPCCALT GEGLAQGFRK LTGFVKSHMK SRGDTLAFFK QN