Recombinant human RAB35 protein
RAB35, also known as ras-related protein Rab-35, is localized to the plasma membrane and endocytic compartments and controls a fast endocytic recycling pathway. Consistent with a key requirement for Rab35-regulated recycling during cell division, inhibition of Rab35 function leads to the accumulation of endocytic markers on numerous cytoplasmic vacuoles in cells that failed cytokinesis. Recombinant human RAB35 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
Ras-related protein Rab-35, H-ray, RAB1C, RAY, RAB35 member RAS oncogene family, GTP-binding protein RAY, Ras-related protein Rab-1C
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
25.1 kDa (221aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Kouranti I., et al. (2006) Curr. Biol. 16:1719-1725; ; Marat A.L., et al. (2010) J. Biol. Chem. 285:10627-10637
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, 0.15M NaCl, 1mM DTT
Scientific Category
Signal Transduction
NCBI Accession Number
NP_006852.1
Uniprot Accession Number
Q15286
Species
Human
AA Sequence
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