Recombinant human RAB35 protein

RAB35, also known as ras-related protein Rab-35, is localized to the plasma membrane and endocytic compartments and controls a fast endocytic recycling pathway. Consistent with a key requirement for Rab35-regulated recycling during cell division, inhibition of Rab35 function leads to the accumulation of endocytic markers on numerous cytoplasmic vacuoles in cells that failed cytokinesis. Recombinant human RAB35 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Ras-related protein Rab-35, H-ray, RAB1C, RAY, RAB35 member RAS oncogene family, GTP-binding protein RAY, Ras-related protein Rab-1C

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

25.1 kDa (221aa) confirmed by MALDI-TOF

Additionnal Information

RAB35, Ras-related protein Rab-35, H-ray, RAB1C, RAY, ATGP1218-10 µg, ATGP1218-20 µg, ATGP1218-50 µg, ATGP1218-100 µg, ATGP1218-250 µg, ATGP1218-500 µg, ATGP1218-1 mg, ATGP1218-10, ATGP1218-20, ATGP1218-50, ATGP1218-100, ATGP1218-250, ATGP1218-500, ATGP1218-1

References & Citations

Kouranti I., et al. (2006) Curr. Biol. 16:1719-1725; ; Marat A.L., et al. (2010) J. Biol. Chem. 285:10627-10637

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, 0.15M NaCl, 1mM DTT

Scientific Category

Signal Transduction

NCBI Accession Number

NP_006852.1

Uniprot Accession Number

Q15286

Species

Human

AA Sequence

MARDYDHLFK LLIIGDSGVG KSSLLLRFAD NTFSGSYITT IGVDFKIRTV EINGEKVKLQ IWDTAGQERF RTITSTYYRG THGVIVVYDV TSAESFVNVK RWLHEINQNC DDVCRILVGN KNDDPERKVV ETEDAYKFAG QMGIQLFETS AKENVNVEEM FNCITELVLR AKKDNLAKQQ QQQQNDVVKL TKNSKRKKRC C