Recombinant human NTH1 protein

NTHL1, also known as nth endonuclease III-like protein 1, is one of the enzyme that has been shown to act on a large number of oxidatively damaged DNA sites. It has an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. It acts on a damaged strand, 5' from the damaged site and incises damaged DNA at cytosines, thymines and guanines. Recombinant human NTHL1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Endonuclease III-like protein 1, NTH1, OCTS3

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 80% by SDS-PAGE

Molecular Weight

36.6 kDa (332aa) confirmed by MALDI-TOF

Additionnal Information

NTH1, Endonuclease III-like protein 1, NTH1, OCTS3, ATGP1217-10 µg, ATGP1217-20 µg, ATGP1217-50 µg, ATGP1217-100 µg, ATGP1217-250 µg, ATGP1217-500 µg, ATGP1217-1 mg, ATGP1217-10, ATGP1217-20, ATGP1217-50, ATGP1217-100, ATGP1217-250, ATGP1217-500, ATGP1217-1

References & Citations

Imai K., et al. (1998) Gene. 222:287-295; ; Ikeda S., et al. (1998) J. Biol. Chem. 273:21585-21593

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, 0.15M NaCl, 1mM DTT

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_002519.1

Uniprot Accession Number

P78549

Species

Human

AA Sequence

MCSPQESGMT ALSARMLTRS RSLGPGAGPR GCREEPGPLR RREAAAEARK SHSPVKRPRK AQRLRVAYEG SDSEKGEGAE PLKVPVWEPQ DWQQQLVNIR AMRNKKDAPV DHLGTEHCYD SSAPPKVRRY QVLLSLMLSS QTKDQVTAGA MQRLRARGLT VDSILQTDDA TLGKLIYPVG FWRSKVKYIK QTSAILQQHY GGDIPASVAE LVALPGVGPK MAHLAMAVAW GTVSGIAVDT HVHRIANRLR WTKKATKSPE ETRAALEEWL PRELWHEING LLVGFGQQTC LPVHPRCHAC LNQALCPAAQ GL