Recombinant human NTH1 protein
NTHL1, also known as nth endonuclease III-like protein 1, is one of the enzyme that has been shown to act on a large number of oxidatively damaged DNA sites. It has an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. It acts on a damaged strand, 5' from the damaged site and incises damaged DNA at cytosines, thymines and guanines. Recombinant human NTHL1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
Endonuclease III-like protein 1, NTH1, OCTS3
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 80% by SDS-PAGE
Molecular Weight
36.6 kDa (332aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Imai K., et al. (1998) Gene. 222:287-295; ; Ikeda S., et al. (1998) J. Biol. Chem. 273:21585-21593
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, 0.15M NaCl, 1mM DTT
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_002519.1
Uniprot Accession Number
P78549
Species
Human
AA Sequence
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