Recombinant human ARF3 protein

ARF3, also known as ADP-ribosylation factor 3, functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. This protein involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus. Recombinant human ARF3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

ADP-ribosylation factor 3

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

22.8 kDa (201aa) confirmed by MALDI-TOF

Additionnal Information

ARF3, ADP-ribosylation factor 3, ATGP1215-10 µg, ATGP1215-20 µg, ATGP1215-50 µg, ATGP1215-100 µg, ATGP1215-250 µg, ATGP1215-500 µg, ATGP1215-1 mg, ATGP1215-10, ATGP1215-20, ATGP1215-50, ATGP1215-100, ATGP1215-250, ATGP1215-500, ATGP1215-1

References & Citations

Bobak D.A., et al. (1989) Proc. Natl. Acad. Sci. u.S.A. 86:6101-6105; ; Takeya R., et al. (2000) Biochem. Biophys. Res. Commun. 267:149-155

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT

Scientific Category

Signal Transduction

NCBI Accession Number

NP_001650

Uniprot Accession Number

P61204

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGNIFGNLLK SLIGKKEMRI LMVGLDAAGK TTILYKLKLG EIVTTIPTIG FNVETVEYKN ISFTVWDVGG QDKIRPLWRH YFQNTQGLIF VVDSNDRERV NEAREELMRM LAEDELRDAV LLVFANKQDL PNAMNAAEIT DKLGLHSLRH RNWYIQATCA TSGDGLYEGL DWLANQLKNK K