Recombinant human ARF3 protein
ARF3, also known as ADP-ribosylation factor 3, functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. This protein involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus. Recombinant human ARF3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
ADP-ribosylation factor 3
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
22.8 kDa (201aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Bobak D.A., et al. (1989) Proc. Natl. Acad. Sci. u.S.A. 86:6101-6105; ; Takeya R., et al. (2000) Biochem. Biophys. Res. Commun. 267:149-155
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT
Scientific Category
Signal Transduction
NCBI Accession Number
NP_001650
Uniprot Accession Number
P61204
Species
Human
AA Sequence
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