Recombinant human GPD1L protein

GPD1L, also known as glycerol-3-phosphate dehydrogenase 1-like protein, converts sn-glycerol 3-phosphate to glycerone phosphate. This protein is found in the cytoplasm, associated with the plasma membrane, where it binds the sodium channel, voltage-gated, type V, alpha subunit (SCN5A) . Mutations in GPD1L are the cause of sudden infant death syndrome (SIDS) and Brugada syndrome type 2, an autosomal dominant tachyarrhythmia. Recombinant human GPD1L protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Glycerol-3-phosphate dehydrogenase 1-like protein, GPD1-L

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

40.6 kDa (371aa) confirmed by MALDI-TOF

Additionnal Information

GPD1L, Glycerol-3-phosphate dehydrogenase 1-like protein, GPD1-L, ATGP1214-10 µg, ATGP1214-20 µg, ATGP1214-50 µg, ATGP1214-100 µg, ATGP1214-250 µg, ATGP1214-500 µg, ATGP1214-1 mg, ATGP1214-10, ATGP1214-20, ATGP1214-50, ATGP1214-100, ATGP1214-250, ATGP1214-500, ATGP1214-1

References & Citations

Valdivia C.R., et al. (2009) Am. J. Physiol. 297:H1446-H1452; ; Van Norstrand D.W., et al. (2007) Circulation. 116:2253-2259

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT

Scientific Category

Metabolism

NCBI Accession Number

NP_055956

Uniprot Accession Number

Q8N335

Species

Human

AA Sequence

MAAAPLKVCI VGSGNWGSAV AKIIGNNVKK LQKFASTVKM WVFEETVNGR KLTDIINNDH ENVKYLPGHK LPENVVAMSN LSEAVQDADL LVFVIPHQFI HRICDEITGR VPKKALGITL IKGIDEGPEG LKLISDIIRE KMGIDISVLM GANIANEVAA EKFCETTIGS KVMENGLLFK ELLQTPNFRI TVVDDADTVE LCGALKNIVA VGAGFCDGLR CGDNTKAAVI RLGLMEMIAF ARIFCKGQVS TATFLESCGV ADLITTCYGG RNRRVAEAFA RTGKTIEELE KEMLNGQKLQ GPQTSAEVYR ILKQKGLLDK FPLFTAVYQI CYESRPVQEM LSCLQSHPEH T