Recombinant human SIT1 protein
SIT1, also known as signaling threshold-regulating transmembrane adapter 1, negatively regulates TCR (T-cell antigen receptor) -mediated signaling in T-cells. This protein involved in positive selection of T-cells. Recombinant human SIT1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
Signaling threshold-regulating transmembrane adapter 1, SIT
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
16.9 kDa (156aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
Additionnal Information
References & Citations
Marie-Cardine A., et al. (1999) J. Exp. Med. 189:1181-1194; ; Pfrepper K., et al. (2001) Eur. J. Immunol. 31:1825-1836
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol
Scientific Category
Immunology
NCBI Accession Number
NP_055265.1
Uniprot Accession Number
Q9Y3P8
Species
Human
AA Sequence
HLSQWTRGR SRSHPGQGRS GESVEEVPLY GNLHYLQTGR LSQDPEPDQQ DPTLGGPARA AEEVMCYTSL QLRPPQGRIP GPGTPVKYSE VVLDSEPKSQ ASGPEPELYA SVCAQTRRAR ASFPDQAYAN SQPAAS
Explore Other Products
Browse additional items from our catalog
