Recombinant human UBD/FAT10 protein
UBD, also known as ubiquitin D, is an ubiquitin-like modifier (UBL) of the ubiquitin protein family. UBD is an ubiquitin-like protein that can form covalent conjugates, thereby targeting proteins for degradation by the 26S proteasome. UBD has putative roles in regulation of cell cycle, innate immunity, and apoptosis. UBD is a TNF-alpha-inducible ubiquitin-like protein with a putative role in immune response. Recombinant human UBD protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
Ubiquitin D, Diubiquitin, FAT10, GABBR1, uBD-3
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
20.9 kDa (188aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Hipp M.S., et al. (2005) Mol. Cell. Biol. 25:3483-3491; ; Ross M.J., et al. (2006) J. Am. Soc. Nephrol. 17:996-1004
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, 0.15M NaCl, 1mM DTT
Scientific Category
Ubiquitination
NCBI Accession Number
NP_006389.2
Uniprot Accession Number
O15205
Species
Human
AA Sequence
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