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Recombinant human CENPH protein

CENPH, also known as centromere protein H, belongs to the centromere protein H family. This protein is component of the CENPA-NAC (nucleosome-associated) complex that plays a central role in assembly of kinetochore proteins. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Recombinant human CENPH protein, fused to His-tag at N-terminus, was expressed in E. coli and denatured using detergent during a conventional chromatography purification process.

Product Specifications

Product Name Alternative

Centromere protein H, CENP H, Centromere protein H, ICEN35, Interphase centromere complex protein 35, Kinetochore protein CENP H, NNF1, MIND kinetochore complex component homolog, PMF1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

15.5 kDa (133aa)

Additionnal Information

CENPH, Centromere protein H, CENP H, Centromere protein H, ICEN35, Interphase centromere complex protein 35, Kinetochore protein CENP H, NNF1, MIND kinetochore complex component homolog, PMF1, ATGP1207-10 µg, ATGP1207-20 µg, ATGP1207-50 µg, ATGP1207-100 µg, ATGP1207-250 µg, ATGP1207-500 µg, ATGP1207-1 mg, ATGP1207-10, ATGP1207-20, ATGP1207-50, ATGP1207-100, ATGP1207-250, ATGP1207-500, ATGP1207-1

References & Citations

Saffery R., et al. (2003) Mol Cell. 12 (2) :509-16.; ; Orthaus S., et al. (2006) Biochem Biophys Res Commun. 15; ;348 (1) :36-46.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Scientific Category

Cancer

NCBI Accession Number

NP_075060

Uniprot Accession Number

Q9H3R5

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MLNKLIMKSQ QESWDLEEKL LDIRKKRLQL KQASESKLLE IQTEKNKQKI DLDSMENSER IKIIRQNLQM EIKITTVIQH VFQNLILGSK VNWAEDPALK EIVLQLEKNV DMM

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