Recombinant human MYL6B protein
MYL6B is a heavy chain regulator found in smooth muscle and non-muscle Myosin complexes. Contractile activity in smooth muscle is regulated by the calcium/calmodulin-dependent phosphorylation of Myosin light chain by Myosin light chain kinase. MYL6B does not bind calcium during contraction. It is primarily found in a hexamer consisting of four light chains and two heavy chains. It most commonly interacts with Myosin Va, an Actin based motor that can move in large steps. MYL6B is expressed in most tissues with neurons and smooth muscle tissue having the highest expression. Recombinant human MYL6B protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Myosin light chain 6B, MLC1SA
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
25.2 kDa (231aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Mitsui T., et al. (1992) J Biol Chem. 267 (23) :16727-35.; ; Takeya K., et al. (2008) Am J Physiol Renal Physiol. 294 (6) :1487-92.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT
Scientific Category
Cytoskeleton
NCBI Accession Number
NP_002466
Uniprot Accession Number
P14649
Species
Human
AA Sequence
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