Recombinant human RGS17 protein

RGS17, also known as regulator of G-protein signaling 17, attenuates the signaling activity of G-proteins by binding to activated, GTP-bound G alpha subunits and acting as a GTPase activating protein (GAP), increasing the rate of conversion of the GTP to GDP. This hydrolysis allows the G alpha subunits to bind G beta/gamma subunit heterodimers, forming inactive G-protein heterotrimers, thereby terminating the signal. It plays an important role in termination of signalling by mu opioid receptors and development of tolerance to opioid analgesic drugs. Recombinant human RGS17 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Regulator of G-protein signaling 17, hRGS17, RGS-17, RGSZ2

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

26.5 kDa (230aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

RGS17, Regulator of G-protein signaling 17, hRGS17, RGS-17, RGSZ2, ATGP1200-10 µg, ATGP1200-20 µg, ATGP1200-50 µg, ATGP1200-100 µg, ATGP1200-250 µg, ATGP1200-500 µg, ATGP1200-1 mg, ATGP1200-10, ATGP1200-20, ATGP1200-50, ATGP1200-100, ATGP1200-250, ATGP1200-500, ATGP1200-1

References & Citations

Sierra D.A. et al. (2002) Genomics 79: 177-185.; ; Mao H. et al. (2004) J. Biol. Chem. 279: 26314-26322.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.1M NaCl,1mM DTT

Scientific Category

Signal Transduction

NCBI Accession Number

NP_036551

Uniprot Accession Number

Q9UGC6

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MRKRQQSQNE GTPAVSQAPG NQRPNNTCCF CWCCCCSCSC LTVRNEERGE NAGRPTHTTK MESIQVLEEC QNPTAEEVLS WSQNFDKMMK APAGRNLFRE FLRTEYSEEN LLFWLACEDL KKEQNKKVIE EKARMIYEDY ISILSPKEVS LDSRVREVIN RNLLDPNPHM YEDAQLQIYT LMHRDSFPRF LNSQIYKSFV ESTAGSSSES