Recombinant human MGP protein
MGP, also known as Matrix Gla protein, is a protein involved in regulating calcification in the extracellular matrix, in particular in cartilage and arteries. MGP is a vitamin K-dependent protein, and contains five to six residues of Gla, a Ca2+ binding amino acid requiring vitamin K-dependent gamma carboxylase for its formation. It is found in human bone extracts, likely formed by COOH-terminal processing by carboxypeptidase B-like enzymatic activity. Recombinant human MGP protein, fused to His-tag at N-terminus, was expressed in E. coli and denatured using detergent during a conventional chromatography purification process.
Product Specifications
Product Name Alternative
Matrix Gla protein isoform 2 precursor, GIG36, MGLAP, NTI
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Denatured
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
11.8 kDa (98aa)
Additionnal Information
References & Citations
Cancela L.et al. (1990) J Biol Chem. 265: 15040-15048.; ; Hale J.E.et al. (1991) J. Biol. Chem. 266: 21145-21149.
Other References
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol
Scientific Category
Cytoskeleton
NCBI Accession Number
NP_000891.2
Uniprot Accession Number
P08493
Species
Human
AA Sequence
YESHESMES YELNPFINRR NANTFISPQQ RWRAKVQERI RERSKPVHEL NREACDDYRL CERYAMVYGY NAAYNRYF
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