Recombinant human MAF1 protein

MAF1 that is similar to Maf1, a Saccharomyces cerevisiae protein is a protein highly conserved in eukaryotic cells and is localized to the nucleus. Interactng with BRF2, MAF1 functions to mediate signals that specifically repress the activity of RNA polymerase III (Pol III), specifically by inhibiting the assembly of TFIIIB onto DNA. It responds to changes in the cellular environment and represses pol III transcription. Recombinant human MAF1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

MAF1 homolog (S. cerevisiae), MGC20332, MGC31779, MGC39758

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

31.0 kDa (276aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

MAF1, MAF1 homolog (S. cerevisiae), MGC20332, MGC31779, MGC39758, ATGP1197-10 µg, ATGP1197-20 µg, ATGP1197-50 µg, ATGP1197-100 µg, ATGP1197-250 µg, ATGP1197-500 µg, ATGP1197-1 mg, ATGP1197-10, ATGP1197-20, ATGP1197-50, ATGP1197-100, ATGP1197-250, ATGP1197-500, ATGP1197-1

References & Citations

Pluta K. et al. (2001) Mol. Cell. Biol. 21: 5031-5040; ; upadhya R. et al. (2002) Mol. Cell. 10: 1489-1494.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

AAH31273

Uniprot Accession Number

Q9H063

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MKLLENSSFE AINSQLTVET GDAHIIGRIE SYSCKMAGDD KHMFKQFCQE GQPHVLEALS PPQTSGLSPS RLSKSQGGEE EGPLSDKCSR KTLFYLIATL NESFRPDYDF STARSHEFSR EPSLSWVVNA VNCSLFSAVR EDFKDLKPQL WNAVDEEICL AECDIYSYNP DLDSDPFGED GSLWSFNYFF YNKRLKRIVF FSCRSISGST YTPSEAGNEL DMELGEEEVE EESRSRGSGA EETSTMEEDR VPVICI