Recombinant human MPI protein

Mannose-6-phosphate isomerase, also known as MPI, belongs to the mannose-6-phosphate isomerase type 1 family, and is expressed in all tissues, more abundantly in heart, brain and skeletal muscle. Localized to the cytoplasm, MPI utilizes zinc as a cofactor and catalyzes the interconversion of fructose-6-phosphate and mannose-6-phosphate. Mutations in the MPI gene were found in patients with carbohydrate-deficient glycoprotein syndrome, type Ib.

Product Specifications

Product Name Alternative

Mannose-6-phosphate isomerase, PMI, PMI1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

41.9 kDa (382aa)

Additionnal Information

MPI, Mannose-6-phosphate isomerase, PMI, PMI1, ATGP1192-10 µg, ATGP1192-20 µg, ATGP1192-50 µg, ATGP1192-100 µg, ATGP1192-250 µg, ATGP1192-500 µg, ATGP1192-1 mg, ATGP1192-10, ATGP1192-20, ATGP1192-50, ATGP1192-100, ATGP1192-250, ATGP1192-500, ATGP1192-1

References & Citations

Schollen E., et al. (2000) Hum Mutat. 16:247-252.; ; Proudfoot A E., et al. (1996) Biochem J. 318:437-442.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 5% glycerol

Scientific Category

Metabolism

NCBI Accession Number

AAH17351

Uniprot Accession Number

P34949

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MAAPRVFPLS CAVQQYAWGK MGSNSEVARL LASSDPLAQI AEDKPYAELW MGTHPRGDAK ILDNRISQKT LSQWIAENQD SLGSKVKDTF NGNLPFLFKV LSVETPLSIQ AHPNKELAEK LHLQAPQHYP DANHKPEMAI ALTPFQGLCG FRPVEEIVTF LKTAAGNNME DIFGELLLQL HQQYPGDIGC FAIYFLNLLT LKPGEAMFLE ANVPHAYLKG DCVECMACSD NTVRAGLTPK FIDVPTLCEM LSYTPSSSKD RLFLPTRSQE DPYLSIYDPP VPDFTIMKTE VPGSVTEYKV LALDSASILL MVQGTVIAST PTTQTPIPLQ RGGVLFIGAN ESVSLKLTEP KDLLIFRACC LL