Recombinant mouse FGF acidic/FGF-1 protein

Fibroblast growth factor 1, also known as FGF1, belongs to the fibroblast growth factor family. It is a potent mitogenic agent. FGF1 has been detected in large amounts in the brain. Other cells known to express FGF1 include hepatocytes, vascular smooth muscle cells, CNS neurons, skeletal muscle cells, fibroblasts, keratinocytes, endothelial cells, intestinal columnar epithelium cells and pituitary basophils and acidophils. The growth factor acts through activation of specific cell-surface receptors leading to intracellular tyrosine phosphorylation cascade. Recombinant mouse FGF1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Fibroblast growth factor 1 (acidic), Dffrx, Fam, Fgf-1, Fgfa, Acidic fibroblast growth factor, aFGF, Heparin-binding growth factor 1, HBGF-1

Expression System

E.coli

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

18 kDa (161aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

FGF1, Fibroblast growth factor 1 (acidic), Dffrx, Fam, Fgf-1, Fgfa, ATGP1189-10 µg, ATGP1189-20 µg, ATGP1189-50 µg, ATGP1189-100 µg, ATGP1189-250 µg, ATGP1189-500 µg, ATGP1189-1 mg, ATGP1189-10, ATGP1189-20, ATGP1189-50, ATGP1189-100, ATGP1189-250, ATGP1189-500, ATGP1189-1

References & Citations

Jaye M., et al. (1986) Science. 233:541-544.; ; Stauber D J., et al. (2000) Proc Natl Acad Sci. 97:49-54

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 30% glycerol, 0.1M NaCl

Scientific Category

Hormones & Cytokines

NCBI Accession Number

NP_034327

Uniprot Accession Number

P61148

Species

Mouse

AA Sequence

FNLPLGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SAGEVYIKGT ETGQYLAMDT EGLLYGSQTP NEECLFLERL EENHYNTYTS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D