Recombinant human ABO protein
ABO, also known as NAGAT, belongs to the glycosyltransferase 6 family. This protein is the basis of the ABO blood group system and related to the first discovered blood group system, ABO. Which allele is present in an individual determines the blood group. The histo-blood group ABO involves three carbohydrate antigens: A, B, and H. A, B, and AB individuals express a glycosyltransferase activity that converts the H antigen to the A antigen (by addition of uDP-GalNAc) or to the B antigen (by addition of uDP-Gal), whereas O individuals lack such activity. Recombinant human ABO protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
37.4 kDa (322aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Persson M., et al. (2007) J. Biol. Chem. 282:9564-9570; ; Nydegger, u.E., et al. (2005) Ann. N.Y. Acad. Sci. 1050: 40-51.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 20% glycerol, 200 mM NaCl
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_065202
Uniprot Accession Number
P16442
Species
Human
AA Sequence
Frequently Asked Questions
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