Recombinant human Crystallin gamma D/CRYGD protein

CRYGD belongs to the beta/gamma-crystallin family. Crystallins are the dominant structural components of the vertebrate eye lens. Mammalian lens crystallins are divided into alpha, beta, and gamma families. Gamma-crystallins have been involved in cataract formation. Defects in CRYGD are a cause of cataract autosomal dominant (ADC), cataract congenital non-nuclear polymorphic autosomal dominant (CCP), cataract congenital cerulean type 3 (CCA3) and cataract crystalline aculeiform (CACA) . Recombinant human CRYGD protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

PCC, Gamma-D-crystallin, Gamma-crystallin D, Gamma-crystallin 4, CTRCT4, cry-g-D, CRYG4, CCP, CCA3, CACA

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by absorbance at 280 nm)

Purity

> 95% by SDS-PAGE

Molecular Weight

22.9 kDa (194a) confirmed by MALDI-TOF

Additionnal Information

PCC, Gamma D crystallin, Gamma-D-crystallin, Gamma crystallin D, Gamma-crystallin D, Gamma crystallin 4, Gamma-crystallin 4, CTRCT4, cry-g-D, CRYG4, CCP, CCA3, CACA, ATGP1184-10 µg, ATGP1184-20 µg, ATGP1184-50 µg, ATGP1184-100 µg, ATGP1184-250 µg, ATGP1184-500 µg, ATGP1184-1 mg, ATGP1184-010, ATGP1184-020, ATGP1184-050, ATGP1184-100, ATGP1184-250, ATGP1184-500, ATGP1184-01M

References & Citations

Pande A., et al. (2001) Proc. Natl. Acad. Sci. u.S.A. 98:6116-6120; ; Plotnikova O.V., et al. (2007) Am. J. Hum. Genet. 81:32-43

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 5mM DTT, 10% glycerol, 200 mM NaCl

Scientific Category

Neuroscience

NCBI Accession Number

NP_008822

Uniprot Accession Number

P07320

Species

Human

AA Sequence

MGKITLYEDR GFQGRHYECS SDHPNLQPYL SRCNSARVDS GCWMLYEQPN YSGLQYFLRR GDYADHQQWM GLSDSVRSCR LIPHSGSHRI RLYEREDYRG QMIEFTEDCS CLQDRFRFNE IHSLNVLEGS WVLYELSNYR GRQYLLMPGD YRRYQDWGAT NARVGSLRRV IDFS