Recombinant human Crystallin gamma D/CRYGD protein
CRYGD belongs to the beta/gamma-crystallin family. Crystallins are the dominant structural components of the vertebrate eye lens. Mammalian lens crystallins are divided into alpha, beta, and gamma families. Gamma-crystallins have been involved in cataract formation. Defects in CRYGD are a cause of cataract autosomal dominant (ADC), cataract congenital non-nuclear polymorphic autosomal dominant (CCP), cataract congenital cerulean type 3 (CCA3) and cataract crystalline aculeiform (CACA) . Recombinant human CRYGD protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
PCC, Gamma-D-crystallin, Gamma-crystallin D, Gamma-crystallin 4, CTRCT4, cry-g-D, CRYG4, CCP, CCA3, CACA
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by absorbance at 280 nm)
Purity
> 95% by SDS-PAGE
Molecular Weight
22.9 kDa (194a) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Pande A., et al. (2001) Proc. Natl. Acad. Sci. u.S.A. 98:6116-6120; ; Plotnikova O.V., et al. (2007) Am. J. Hum. Genet. 81:32-43
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 5mM DTT, 10% glycerol, 200 mM NaCl
Scientific Category
Neuroscience
NCBI Accession Number
NP_008822
Uniprot Accession Number
P07320
Species
Human
AA Sequence
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