Recombinant human CLF-1/CLC protein

CLCF1, also known as cardiotrophin-like cytokine factor 1, belongs to the interleukin 6 family of cytokines, which are involved in cell signaling through phosphorylation of gp130. CLCF1 has a sequence of 225-amino acids with a 27-aa signal peptide, a molecular mass of 22 kDa in mature form, and the highest homology to cardiotrophin-1 and ciliary neurotrophic factor. CLCF1 can be actively secreted from cells by forming a complex with soluble type I CRLF1 or soluble CNTFR. Defects in CLCF1 cause cold-induced sweating syndrome 2 (CISS2) . This syndrome is characterized by a profuse sweating after exposure to cold as well as congenital physical abnormalities of the head and spine.

Product Specifications

Product Name Alternative

Cardiotrophin-like cytokine factor 1, BSF-3, BSF3, CISS2, CLC, NNT-1, NNT1, NR6

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

24.6 kDa (219aa)

Additionnal Information

CLF1, CLC, Cardiotrophin-like cytokine factor 1, BSF-3, BSF3, CISS2, CLC, NNT-1, NNT1, NR6, ATGP1179-10 µg, ATGP1179-20 µg, ATGP1179-50 µg, ATGP1179-100 µg, ATGP1179-250 µg, ATGP1179-500 µg, ATGP1179-1 mg, ATGP1179-10, ATGP1179-20, ATGP1179-50, ATGP1179-100, ATGP1179-250, ATGP1179-500, ATGP1179-1

References & Citations

Senaldi G., et al. (1999) Proc. Natl. Acad. Sci. u.S.A. 96:11458-11463; ; Shi Y., et al. (1999) Biochem. Biophys. Res. Commun. 262:132-138

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM sodium citrate (pH 3.5) 0.4M urea, 10% glycerol

Scientific Category

Immunology

NCBI Accession Number

NP_037378

Uniprot Accession Number

Q9UBD9

Species

Human

AA Sequence

LNRTGDPGP GPSIQKTYDL TRYLEHQLRS LAGTYLNYLG PPFNEPDFNP PRLGAETLPR ATVDLEVWRS LNDKLRLTQN YEAYSHLLCY LRGLNRQAAT AELRRSLAHF CTSLQGLLGS IAGVMAALGY PLPQPLPGTE PTWTPGPAHS DFLQKMDDFW LLKELQTWLW RSAKDFNRLK KKMQPPAAAV TLHLGAHGF