Recombinant human CYB5A protein
CYB5A, also known as cytochrome b5 isoform 1, belongs to the cytochrome b5 family. Cytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases. Recombinant human CYB5A protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
Cytochrome b5, CYB5, MCB5
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
14.9 kDa (132aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Liu H., et al. (1994) Hum. Genet. 93:568-570; ; Abe K., et al. (1985) J. Biochem. 97:1659-1668
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl
Scientific Category
Metabolism
NCBI Accession Number
NP_683725.1
Uniprot Accession Number
P00167
Species
Human
AA Sequence
MAEQSD EAVKYYTLEE IQKHNHSKST WLILHHKVYD LTKFLEEHPG GEEVLREQAG GDATENFEDV GHSTDAREMS KTFIIGELHP DDRPKLNKPP ETLITTIDSS SS
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