Recombinant e.coli rnhA protein

RnhA is an endonuclease that specifically degrades the RNA of RNA-DNA hybrids. Localized to the nucleus, this protein mediates the removal of Okazaki fragment RNA primers that are present on the lagging strand during DNA replication. rnhA catalyzes the endonucleolytic cleavage of RNA to a 5'-phosphomonoester and is able to bind magnesium or manganese as cofactors. Recombinant e. coli rnhA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Ribonuclease HI degrades RNA of DNA-RNA hybrids., Ribonuclease HI, degrades RNA of DNA-RNA hybrids., cer, dasF, ECK0214, herA, JW0204, rnh, sdrA, sin

Expression System

E.coli

Antigen Species

E.coli

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

20 kDa (178aa) confirmed by MALDI-TOF

Additionnal Information

RnhA, Ribonuclease HI degrades RNA of DNA-RNA hybrids., Ribonuclease HI, degrades RNA of DNA-RNA hybrids., cer, dasF, ECK0214, herA, JW0204, rnh, sdrA, sin, ATGP1173-10 µg, ATGP1173-20 µg, ATGP1173-50 µg, ATGP1173-100 µg, ATGP1173-250 µg, ATGP1173-500 µg, ATGP1173-1 mg, ATGP1173-10, ATGP1173-20, ATGP1173-50, ATGP1173-100, ATGP1173-250, ATGP1173-500, ATGP1173-1

References & Citations

Nowotny M., et al. (2007) Mol Cell. 28 (2) :264-76.; ; Ten Asbroek AL., et al. (2002) Eur J Biochem. 269 (2) :583-92.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_414750

Uniprot Accession Number

P0A7Y4

Species

E.coli

AA Sequence

MLKQVEI FTDGSCLGNP GPGGYGAILR YRGREKTFSA GYTRTTNNRM ELMAAIVALE ALKEHCEVIL STDSQYVRQG ITQWIHNWKK RGWKTADKKP VKNVDLWQRL DAALGQHQIK WEWVKGHAGH PENERCDELA RAAAMNPTLE DTGYQVEV