Recombinant e.coli rnhA protein
RnhA is an endonuclease that specifically degrades the RNA of RNA-DNA hybrids. Localized to the nucleus, this protein mediates the removal of Okazaki fragment RNA primers that are present on the lagging strand during DNA replication. rnhA catalyzes the endonucleolytic cleavage of RNA to a 5'-phosphomonoester and is able to bind magnesium or manganese as cofactors. Recombinant e. coli rnhA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Ribonuclease HI degrades RNA of DNA-RNA hybrids., Ribonuclease HI, degrades RNA of DNA-RNA hybrids., cer, dasF, ECK0214, herA, JW0204, rnh, sdrA, sin
Expression System
E.coli
Antigen Species
E.coli
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
20 kDa (178aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Nowotny M., et al. (2007) Mol Cell. 28 (2) :264-76.; ; Ten Asbroek AL., et al. (2002) Eur J Biochem. 269 (2) :583-92.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_414750
Uniprot Accession Number
P0A7Y4
Species
E.coli
AA Sequence
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