Recombinant human QDPR protein

QDPR is a member of the short-chain dehydrogenases/reductase (SDR) family of enzymes. Functioning as a homodimer, QDPR plays an important role in the recycling of tetrahydrobiopterin (BH4), an essential cofactor for the hydroxylation of the aromatic amino acids (tryptophan, tyrosine and phenylalanine) . More specifically, QDPR catalyzes the regeneration of BH4 from quinonoid dihydrobiopterin (qBH2), the product generated from the hydroxylation reactions. Mutations in the gene encoding QDPR can lead to phenylketonuria II. Recombinant human QDPR protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Quinoid dihydropteridine reductase., DHPR, FLJ42391, PKu2, SDR33C1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

28.2 kDa (267aa) confirmed by MALDI-TOF

Additionnal Information

QDPR, Quinoid dihydropteridine reductase., DHPR, FLJ42391, PKu2, SDR33C1, ATGP1172-10 µg, ATGP1172-20 µg, ATGP1172-50 µg, ATGP1172-100 µg, ATGP1172-250 µg, ATGP1172-500 µg, ATGP1172-1 mg, ATGP1172-10, ATGP1172-20, ATGP1172-50, ATGP1172-100, ATGP1172-250, ATGP1172-500, ATGP1172-1

References & Citations

Lye LF., et al. (2002) J Biol Chem. 277 (41) :38245-53.; ; Kalkanoqlu HS., et al. (2001) Prenat Diagn. 21 (10) :868-70.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Metabolism

NCBI Accession Number

NP_000311

Uniprot Accession Number

P09417

Species

Human

AA Sequence

MAAAAAA GEARRVLVYG GRGALGSRCV QAFRARNWWV ASVDVVENEE ASASIIVKMT DSFTEQADQV TAEVGKLLGE EKVDAILCVA GGWAGGNAKS KSLFKNCDLM WKQSIWTSTI SSHLATKHLK EGGLLTLAGA KAALDGTPGM IGYGMAKGAV HQLCQSLAGK NSGMPPGAAA IAVLPVTLDT PMNRKSMPEA DFSSWTPLEF LVETFHDWIT GKNRPSSGSL IQVVTTEGRT ELTPAYF