Recombinant human PCNP protein

PCNP (PEST proteolytic signal containing nuclear protein) is a nuclear protein implied to play a role in cell cycle regulation and tumorigenesis. This protein is ubiquitinated post-translationally by NIRF, an ubiquitin ligase. PCNP exsists as three isoforms produced by alternative splicing events and may be involved in cell cycle regulation. Recombinant human PCNP protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

PEST proteolytic signal containing nuclear protein., DKFZp781I24156

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by BCA assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

21.5 kDa (202aa) confirmed by MALDI-TOF

Additionnal Information

PCNP, PEST proteolytic signal containing nuclear protein., DKFZp781I24156, ATGP1171-10 µg, ATGP1171-20 µg, ATGP1171-50 µg, ATGP1171-100 µg, ATGP1171-250 µg, ATGP1171-500 µg, ATGP1171-1 mg, ATGP1171-10, ATGP1171-20, ATGP1171-50, ATGP1171-100, ATGP1171-250, ATGP1171-500, ATGP1171-1

References & Citations

Mori T., et al. (2004) FEBS Lett. 557 (1-3) :209-14.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.5) containing 10% glycerol

Scientific Category

Cell Cycle

NCBI Accession Number

NP_065090.1

Uniprot Accession Number

Q8WW12

Species

Human

AA Sequence

MADGKA GDEKPEKSQR AGAAGGPEEE AEKPVKTKTV SSSNGGESSS RSAEKRSAEE EAADLPTKPT KISKFGFAIG SQTTKKASAI SIKLGSSKPK ETVPTLAPKT LSVAAAFNED EDSEPEEMPP EAKMRMKNIG RDTPTSAGPN SFNKGKHGFS DNQKLWERNI KSHLGNVHDQ DN