Recombinant human PCNP protein
PCNP (PEST proteolytic signal containing nuclear protein) is a nuclear protein implied to play a role in cell cycle regulation and tumorigenesis. This protein is ubiquitinated post-translationally by NIRF, an ubiquitin ligase. PCNP exsists as three isoforms produced by alternative splicing events and may be involved in cell cycle regulation. Recombinant human PCNP protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
PEST proteolytic signal containing nuclear protein., DKFZp781I24156
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by BCA assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
21.5 kDa (202aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Mori T., et al. (2004) FEBS Lett. 557 (1-3) :209-14.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.5) containing 10% glycerol
Scientific Category
Cell Cycle
NCBI Accession Number
NP_065090.1
Uniprot Accession Number
Q8WW12
Species
Human
AA Sequence
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