Recombinant human RGS5 protein

RGS5, also known as regulator of G-protein signaling 5, binds directly to activated G alpha subunits and act as GTPase-activating proteins (GAPs) to attenuate and/or modulate hormone and neurotransmitter receptor-initiated signaling by both G alpha-GTP and G beta gamma. Vascular endothelial cells express the RGS protein RGS5, where it correlates with capillary morphogenesis, thus rendering it a candidate gene involved in capillary growth, angiogenesis, and also potentially the pathophysiology of stroke. Recombinant human RGS5 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Regulator of G-protein signaling 5, MST092, MST106, MST129, MSTP032, MSTP092, MSTP106, MSTP129

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

23.5 kDa (205aa) confirmed by MALDI-TOF

Additionnal Information

RGS5, Regulator of G-protein signaling 5, MST092, MST106, MST129, MSTP032, MSTP092, MSTP106, MSTP129, ATGP1170-10 µg, ATGP1170-20 µg, ATGP1170-50 µg, ATGP1170-100 µg, ATGP1170-250 µg, ATGP1170-500 µg, ATGP1170-1 mg, ATGP1170-10, ATGP1170-20, ATGP1170-50, ATGP1170-100, ATGP1170-250, ATGP1170-500, ATGP1170-1

References & Citations

Hepler J.R. et al. (1999) Trends Pharmacol. Sci. 20: 376-382.; ; Wieland T. et al. (2003) Pharmacol. Ther. 97: 95-115.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl,1mM DTT

Scientific Category

Signal Transduction

NCBI Accession Number

NP_003608

Uniprot Accession Number

O15539

Species

Human

AA Sequence

MCKGLA ALPHSCLERA KEIKIKLGIL LQKPDSVGDL VIPYNEKPEK PAKTQKTSLD EALQWRDSLD KLLQNNYGLA SFKSFLKSEF SEENLEFWIA CEDYKKIKSP AKMAEKAKQI YEEFIQTEAP KEVNIDHFTK DITMKNLVEP SLSSFDMAQK RIHALMEKDS LPRFVRSEFY QELIK