Recombinant human Hydroxyacid Oxidase-1/HAO-1 protein
HAO1, also known as glycolate oxidase, is a member of the superfamily of the alpha hydroxy acid oxidases (HAO) enzymes. It catalyzes the FMN mediated oxidation of glycolate to glyoxylate and glyoxylate to oxalate with reduction of oxygen to hydrogen peroxide. It is most highly expressed in liver and pancreas and is most active on twocarbon substrates such as glycolate. Recently, it has been identified as a major contributor to hyperoxaluria, a disorder in which large deposits of calcium oxalate form kidney stones. Recombinant human HAO1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
Hydroxyacid oxidase 1, GOX (glycolate oxidase), GOX1, HAOX1
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
45.0 kDa (406aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Pennati A. and G. Gadda. (2009) J. Biol. Chem. 284:31214; ; Murray M.S. et al. (2008) Biochemistry, 47:2439
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.5M NaCl
Scientific Category
Metabolism
NCBI Accession Number
NP_060015
Uniprot Accession Number
Q9UJM8
Species
Human
AA Sequence
Frequently Asked Questions
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