Recombinant human Crystallin beta A4/CRYBA4 protein

CRYBA4, also known as Beta-crystallin A4, belongs to the beta/gamma-crystallin family that are the dominant structural components of the vertebrate eye lens. Beta-crystallins form aggregates of different sizes and are able to self-associate to form dimers or to form heterodimers with other beta-crystallins. This gene, a beta acidic group member, is part of a gene cluster with beta-B1, beta-B2, and beta-B3. Recombinant human CRYBA4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Beta-crystallin A4, MCOPCT4, Beta-A4 crystallin

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

24.5 kDa (216aa) confirmed by MALDI-TOF

Additionnal Information

CRYBA4, Beta-crystallin A4, MCOPCT4, ATGP1167-10 µg, ATGP1167-20 µg, ATGP1167-50 µg, ATGP1167-100 µg, ATGP1167-250 µg, ATGP1167-500 µg, ATGP1167-1 mg, ATGP1167-10, ATGP1167-20, ATGP1167-50, ATGP1167-100, ATGP1167-250, ATGP1167-500, ATGP1167-1

References & Citations

Lampi KJ. et al. (1997) J Biol Chem. 272:2268-2275.; ; Billingsley G. et al. (2006) Am J Hum Genet. 79:702-709

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl,1mM DTT

Scientific Category

Heat Shock Proteins

NCBI Accession Number

NP_001877

Uniprot Accession Number

P53673

Species

Human

AA Sequence

MTLQCTKSAG PWKMVVWDED GFQGRRHEFT AECPSVLELG FETVRSLKVL SGAWVGFEHA GFQGQQYILE RGEYPSWDAW GGNTAYPAER LTSFRPAACA NHRDSRLTIF EQENFLGKKG ELSDDYPSLQ AMGWEGNEVG SFHVHSGAWV CSQFPGYRGF QYVLECDHHS GDYKHFREWG SHAPTFQVQS IRRIQQ