Recombinant human KARS protein
KARS, also known as Lysyl-tRNA synthetase, belongs to the class-II aminoacyl-tRNA synthetase family. It exists as both mitochondrial and cytoplasmic isoforms produced by alternative splicing, and thought to play a role in autoimmune diseases, such as polymyositis or dermatomyositis. This protein functions to catalyze the aminoacylation of tRNAs by their corresponding amino acids, thus linking amino acids with tRNA-contained nucleotide triplets. Recombinant human KARS protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
Lysyl-tRNA synthetase, CMTRIB, KARS2, KIAA0070, KRS
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
63.7 kDa (558aa)
Additionnal Information
References & Citations
Targoff I.N. et al. (1993) J. Clin. Invest. 91: 2556-2564.; ; Tolkunova E. et al. (2000) J. Biol. Chem. 275: 35063-35069.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl,1mM DTT
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_005539.1
Uniprot Accession Number
Q15046
Species
Human
AA Sequence
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