Recombinant human TFB1M protein
TFB1M belongs to the methyltransferase superfamily. TFB1M is a dimethyltransferase that methylates mitochondrial 12S rRNA at the conserved stem loop. This protein also is part of the basal mitochondrial transcription complex and is necessary for mitochondrial gene expression. TFB1M stimulates transcription independently of the methyltransferase activity. Recombinant human TFB1M protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
Dimethyladenosine transferase 1, TFB1M (Mitochondrial transcription factor B1)
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
38.8 kDa (340aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
McCulloch V., et al. (2002) Mol. Cell. Biol. 22:1116-1125; ; Gleyzer N., et al. (2005) Mol. Cell. Biol. 25 (4) :1354-66
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_057104
Uniprot Accession Number
Q8WVM0
Species
Human
AA Sequence
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