Recombinant human DCPS protein
DCPS, also known as scavenger mRNA-decapping enzyme, is belongs to the HIT family. It is necessary for the complete degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. A scavenger decapping enzyme, DcpS was shown to hydrolyze the residual m7GpppN cap structure after the complete 3'-5' degradation of the mRNA by the exosome. DcpS releases m7GMP and is unable to cleave cap structures attached to a long RNA chain. Recombinant human DCPS protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
Scavenger mRNA-decapping enzyme DcpS, DCS1, HINT5
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
40.7 kDa (357aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Liu H., et al. (2002) EMBO J. 21:4699-4708; ; Kwasnicka D.A., et al. (2003) J. Biol. Chem. 278:39051-39058
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_054745
Uniprot Accession Number
Q96C86
Species
Human
AA Sequence
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