Recombinant human DCPS protein

DCPS, also known as scavenger mRNA-decapping enzyme, is belongs to the HIT family. It is necessary for the complete degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. A scavenger decapping enzyme, DcpS was shown to hydrolyze the residual m7GpppN cap structure after the complete 3'-5' degradation of the mRNA by the exosome. DcpS releases m7GMP and is unable to cleave cap structures attached to a long RNA chain. Recombinant human DCPS protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Scavenger mRNA-decapping enzyme DcpS, DCS1, HINT5

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

40.7 kDa (357aa) confirmed by MALDI-TOF

Additionnal Information

DCPS, Scavenger mRNA-decapping enzyme DcpS, DCS1, HINT5, ATGP1162-10 µg, ATGP1162-20 µg, ATGP1162-50 µg, ATGP1162-100 µg, ATGP1162-250 µg, ATGP1162-500 µg, ATGP1162-1 mg, ATGP1162-10, ATGP1162-20, ATGP1162-50, ATGP1162-100, ATGP1162-250, ATGP1162-500, ATGP1162-1

References & Citations

Liu H., et al. (2002) EMBO J. 21:4699-4708; ; Kwasnicka D.A., et al. (2003) J. Biol. Chem. 278:39051-39058

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_054745

Uniprot Accession Number

Q96C86

Species

Human

AA Sequence

MADAAPQLGK RKRELDVEEA HAASTEEKEA GVGNGTCAPV RLPFSGFRLQ KVLRESARDK IIFLHGKVNE ASGDGDGEDA VVILEKTPFQ VEQVAQLLTG SPELQLQFSN DIYSTYHLFP PRQLNDVKTT VVYPATEKHL QKYLRQDLRL IRETGDDYRN ITLPHLESQS LSIQWVYNIL DKKAEADRIV FENPDPSDGF VLIPDLKWNQ QQLDDLYLIA ICHRRGIRSL RDLTPEHLPL LRNILHQGQE AILQRYRMKG DHLRVYLHYL PSYYHLHVHF TALGFEAPGS GVERAHLLAE VIENLECDPR HYQQRTLTFA LRADDPLLKL LQEAQQS