Recombinant human PFDN5 protein

PFDN5 belongs to the prefoldin alpha subunit family. Prefoldin (PFDN), a ubiquitously expressed heterohexameric co-chaperone, is necessary for proper folding of nascent proteins, in particular, tubulin and actin. PFDN5 is one of six subunits of prefoldin, a molecular chaperone complex that binds and stabilizes newly synthesized polypeptides, thereby allowing them to fold correctly. This protein may also repress the transcriptional activity of the proto-oncogene c-Myc. Recombinant human PFDN5 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Prefoldin subunit 5 isoform alpha, MM-1, MM1, PFD5

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

19.5 kDa (174aa) confirmed by MALDI-TOF

Additionnal Information

PFDN5, prefoldin subunit 5 isoform alpha, MM-1, MM1, PFD5, ATGP1161-10 µg, ATGP1161-20 µg, ATGP1161-50 µg, ATGP1161-100 µg, ATGP1161-250 µg, ATGP1161-500 µg, ATGP1161-1 mg, ATGP1161-10, ATGP1161-20, ATGP1161-50, ATGP1161-100, ATGP1161-250, ATGP1161-500, ATGP1161-1

References & Citations

Vainberg I.E., et al. (1998) Cell 93:863-873 ; ; Mori K, et al. (1998) J Biol Chem 273 (45) : 29794-800.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 10% glycerol

Scientific Category

Heat Shock Proteins

NCBI Accession Number

NP_002615

Uniprot Accession Number

Q99471

Species

Human

AA Sequence

MAQSINITEL NLPQLEMLKN QLDQEVEFLS TSIAQLKVVQ TKYVEAKDCL NVLNKSNEGK ELLVPLTSSM YVPGKLHDVE HVLIDVGTGY YVEKTAEDAK DFFKRKIDFL TKQMEKIQPA LQEKHAMKQA VMEMMSQKIQ QLTALGAAQA TAKA