Recombinant human NAA10 protein

NAA10 belongs to the acetyltransferase family. NAA10 interacts NAA15, HIF-1 with the ribosome. In its binding to HIF-1, NAA10 acts as a protein acetyltransferase by regulating its stability. In many cell lines, NAA10 is downregulated in response to hypoxia. NAA10 is expressed throughout the development of the brain. Recombinant human NAA10 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

N (alpha) -acetyltransferase 10 NatA catalytic subunit, N (alpha) -acetyltransferase 10, NatA catalytic subunit, ARD1, ARD1A, DXS707, MGC71248, TE2

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

28.6 kDa (255aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

NAA, N (alpha) -acetyltransferase 10 NatA catalytic subunit, N (alpha) -acetyltransferase 10, NatA catalytic subunit, ARD1, ARD1A, DXS707, MGC71248, TE2, ATGP1159-10 µg, ATGP1159-20 µg, ATGP1159-50 µg, ATGP1159-100 µg, ATGP1159-250 µg, ATGP1159-500 µg, ATGP1159-1 mg, ATGP1159-10, ATGP1159-20, ATGP1159-50, ATGP1159-100, ATGP1159-250, ATGP1159-500, ATGP1159-1

References & Citations

Arnesen T., et al. (2005) Biochem. J. 386:433-443; ; Jeong J.-W., et al. (2002) Cell 111:709-720

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 5mM DTT, 10% glycerol, 200 mM NaCl

Scientific Category

Metabolism

NCBI Accession Number

NP_003482

Uniprot Accession Number

P41227

Species

Human

AA Sequence

MNIRNARPED LMNMQHCNLL CLPENYQMKY YFYHGLSWPQ LSYIAEDENG KIVGYVLAKM EEDPDDVPHG HITSLAVKRS HRRLGLAQKL MDQASRAMIE NFNAKYVSLH VRKSNRAALH LYSNTLNFQI SEVEPKYYAD GEDAYAMKRD LTQMADELRR HLELKEKGRH VVLGAIENKV ESKGNSPPSS GEACREEKGL AAEDSGGDSK DLSEVSETTE STDVKDSSEA SDSAS