Recombinant human RGS21 protein

Regulator of G-protein signaling 21, also known as RGS21, is a RGS family member and regulatory molecules that act as GTPase activating proteins for G alpha subunits of heterotrimeric G proteins. This protein inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Recombinant human RGS21 human, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Regulator of G-protein signaling 21

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

20.2 kDa (176aa) confirmed by MALDI-TOF

Additionnal Information

RGS21, Regulator of G-protein signaling 21, ATGP1157-10 µg, ATGP1157-20 µg, ATGP1157-50 µg, ATGP1157-100 µg, ATGP1157-250 µg, ATGP1157-500 µg, ATGP1157-1 mg, ATGP1157-10, ATGP1157-20, ATGP1157-50, ATGP1157-100, ATGP1157-250, ATGP1157-500, ATGP1157-1

References & Citations

Li X., et al. (2005) Acta Biochim Pol. 52 (4) :943-6.; ; Von Buchholtz L., et al. (2004) Eur J Neurosci. 19 (6) :1535-44.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 30% glycerol, 0.1M NaCl

Scientific Category

Signal Transduction

NCBI Accession Number

NP_001034241

Uniprot Accession Number

Q2M5E4

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSHMPVKCC FYRSPTAETM TWSENMDTLL ANQAGLDAFR IFLKSEFSEE NVEFWLACED FKKTKNADKI ASKAKMIYSE FIEADAPKEI NIDFGTRDLI SKNIAEPTLK CFDEAQKLIY CLMAKDSFPR FLKSEIYKKL VNSQQVPNHK KWLPFL