Recombinant human ARL8/ARL5B protein

ADP-ribosylation factor-like protein 5B, also known as ARL5B, belongs to a family of proteins that are structurally similar to ADP-ribosylation factors. ARLs and ARFs are part of the RAS superfamily of regulatory GTPases. ARL5B is most closely related to ARL5, with which it shares 80% sequence identity. Human ARL5B shares 100% identity with mouse ARL8 and 71% identity with the Drosophila homolog. Two isoforms of ARL8 exist as a result of alternative splicing events. Recombinant human ARL5B protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

ADP-ribosylation factor-like protein 5B, ARL8, ADP ribosylation factor like GTPase 5B, ADP-ribosylation factor-like 8

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

22.5 kDa (199aa) confirmed by MALDI-TOF

Additionnal Information

ARL5B, ADP-ribosylation factor-like protein 5B, ARL8, ATGP1155-10 µg, ATGP1155-20 µg, ATGP1155-50 µg, ATGP1155-100 µg, ATGP1155-250 µg, ATGP1155-500 µg, ATGP1155-1 mg, ATGP1155-10, ATGP1155-20, ATGP1155-50, ATGP1155-100, ATGP1155-250, ATGP1155-500, ATGP1155-1

References & Citations

Kahn R A., et al. (2006) J Biol Chem. 172:645-650.; ; Swbald E., et al. (2003 Gene. 311:147-151.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 30% glycerol, 0.1M NaCl, 1mM EDTA

Scientific Category

Signal Transduction

NCBI Accession Number

NP_848930

Uniprot Accession Number

Q96KC2

Species

Human

AA Sequence

MGLIFAKLWS LFCNQEHKVI IVGLDNAGKT TILYQFLMNE VVHTSPTIGS NVEEIVVKNT HFLMWDIGGQ ESLRSSWNTY YSNTEFIILV VDSIDRERLA ITKEELYRML AHEDLRKAAV LIFANKQDMK GCMTAAEISK YLTLSSIKDH PWHIQSCCAL TGEGLCQGLE WMTSRIGVR