Recombinant human PDGF R alpha protein

PDGFRa, also known as platelet derived growth factor receptor alpha, is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. This protein can bind to both A and B subunits of PDGF. It is particularly important for kidney development since mice heterozygous for the receptor exhibit defective kidney phenotypes. Recombinant human PDGFRa protein, fused to His-tag at N-terminus, was expressed in E. coli and purifed by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Product Specifications

Product Name Alternative

Platelet-derived growth factor receptor alpha precursor, CD140A, PDGFR2, MGC74795, Rhe-PDGFRA

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

58.4 kDa (522aa)

Additionnal Information

PDGF R a, Platelet-derived growth factor receptor alpha precursor, CD140A, PDGFR2, MGC74795, Rhe-PDGFRA, ATGP1153-10 µg, ATGP1153-20 µg, ATGP1153-50 µg, ATGP1153-100 µg, ATGP1153-250 µg, ATGP1153-500 µg, ATGP1153-1 mg, ATGP1153-10, ATGP1153-20, ATGP1153-50, ATGP1153-100, ATGP1153-250, ATGP1153-500, ATGP1153-1

References & Citations

Yu J. et al. (2000) J Biol Chem. 275: 19076-82; ; Xie J. et al. (2001) . Proc. Natl. Acad. Sci. uSA 98: 9255-59

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl,1mM DTT

Scientific Category

Cancer

NCBI Accession Number

NP_006197

Uniprot Accession Number

P16234

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MQLSLPSILP NENEKVVQLN SSFSLRCFGE SEVSWQYPMS EEESSDVEIR NEENNSGLFV TVLEVSSASA AHTGLYTCYY NHTQTEENEL EGRHIYIYVP DPDVAFVPLG MTDYLVIVED DDSAIIPCRT TDPETPVTLH NSEGVVPASY DSRQGFNGTF TVGPYICEAT VKGKKFQTIP FNVYALKATS ELDLEMEALK TVYKSGETIV VTCAVFNNEV VDLQWTYPGE VKGKGITMLE EIKVPSIKLV YTLTVPEATV KDSGDYECAA RQATREVKEM KKVTISVHEK GFIEIKPTFS QLEAVNLHEV KHFVVEVRAY PPPRISWLKN NLTLIENLTE ITTDVEKIQE IRYRSKLKLI RAKEEDSGHY TIVAQNEDAV KSYTFELLTQ VPSSILDLVD DHHGSTGGQT VRCTAEGTPL PDIEWMICKD IKKCNNETSW TILANNVSNI ITEIHSRDRS TVEGRVTFAK VEETIAVRCL AKNLLGAENR ELKLVAPTLR SE