Recombinant human RND1 protein

RND1, also known as Rho 6, is Rho-related GTPases form a distinct branch of the Rho family, since they differ from other Rho proteins in size, charge, and biochemical properties. It appears to be constitutively in the activated GTP-bound form and acts as negative regulators of actin assembly and of cell adhesion. It contributes to regulating the organization of the actin cytoskeleton in response to extracellular growth factors. Recombinant human RND1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Rho-related GTP-binding protein Rho6, ARHS, FLJ42294, RHO6, RHO

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

24.5 kDa (220aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

RND1, Rho-related GTP-binding protein Rho6, ARHS, FLJ42294, RHO6, RHO, ATGP1148-10 µg, ATGP1148-20 µg, ATGP1148-50 µg, ATGP1148-100 µg, ATGP1148-250 µg, ATGP1148-500 µg, ATGP1148-1 mg, ATGP1148-10, ATGP1148-20, ATGP1148-50, ATGP1148-100, ATGP1148-250, ATGP1148-500, ATGP1148-1

References & Citations

Nobes C.D. et al. (1998) J. Cell Biol. 141: 187-197.; ; Garrett M.D. et al. (1989) J. Biol. Chem. 264: 10-13.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 30% glycerol, 0.1M NaCl,1mM DTT

Scientific Category

Signal Transduction

NCBI Accession Number

NP_055285.1

Uniprot Accession Number

Q92730

Species

Human

AA Sequence

MKERRAPQPV VARCKLVLVG DVQCGKTAML QVLAKDCYPE TYVPTVFENY TACLETEEQR VELSLWDTSG SPYYDNVRPL CYSDSDAVLL CFDISRPETV DSALKKWRTE ILDYCPSTRV LLIGCKTDLR TDLSTLMELS HQKQAPISYE QGCAIAKQLG AEIYLEGSAF TSEKSIHSIF RTASMLCLNK PSPLPQKSPV