Recombinant human UbcH5b/UBE2D2 protein
UBE2D2 is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme functions in the ubiquitination of the tumor-suppressor protein p53, which is induced by an E3 ubiquitin-protein ligase. uBE2D2 catalyzes ubiquitination of IkB-alpha in a phosphorylation and SCFB-TRCP dependent manner. Recombinant human uBE2D2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
18.9 kDa (167aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Ciechanover A., et al. (1994) FASEB J. 8 (2) :182-19.; ; Orian A., et al. (1995) J Biol Chem. 270 (37) :21707-14.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.1M NaCl.
Scientific Category
Ubiquitination
NCBI Accession Number
NP_003330
Uniprot Accession Number
P62837
Species
Human
AA Sequence
Frequently Asked Questions
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