Recombinant human RGS10 protein

Regulator of G protein signaling (RGS) family members are regulatory molecules that act as GTPase activating proteins (GAPs) for G alpha subunits of heterotrimeric G proteins. RGS proteins are able to deactivate G protein subunits of the Gi alpha, Go alpha and Gq alpha subtypes. They drive G proteins into their inactive GDP-bound forms. Regulator of G protein signaling 10 (RGS10) belongs to this family. All RGS proteins share a conserved 120-amino acid sequence termed the RGS domain. This protein associates specifically with the activated forms of the two related G-protein subunits, G-alphai3 and G-alphaz but fails to interact with the structurally and functionally distinct G-alpha subunits. RGS10 protein is localized in the nucleus. Recombinant human RGS10 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Regulator of G-protein signaling 10, Regulator of G protein signaling 10

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

23.7 kDa (205aa) confirmed by MALDI-TOF

Additionnal Information

RGS10, Regulator of G-protein signaling 10, Regulator of G protein signaling 10, ATGP1144-10 µg, ATGP1144-20 µg, ATGP1144-50 µg, ATGP1144-100 µg, ATGP1144-250 µg, ATGP1144-500 µg, ATGP1144-1 mg, ATGP1144-10, ATGP1144-20, ATGP1144-50, ATGP1144-100, ATGP1144-250, ATGP1144-500, ATGP1144-1

References & Citations

Cali J J., et al. (1992) J Biol Chem. 267:24023-24027.; ; Simon M I., et al. (1991) Science. 252:802-808.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl

Scientific Category

Signal Transduction

NCBI Accession Number

NP_001005339

Uniprot Accession Number

O43665

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSHMFNRAV SRLSRKRPPS DIHDSDGSSS SSHQSLKSTA KWAASLENLL EDPEGVKRFR EFLKKEFSEE NVLFWLACED FKKMQDKTQM QEKAKEIYMT FLSSKASSQV NVEGQSRLNE KILEEPHPLM FQKLQDQIFN LMKYDSYSRF LKSDLFLKHK RTEEEEEDLP DAQTAAKRAS RIYNT