Recombinant human Nanog protein

Nanog homeobox, also known as nanog, is a member of the homeobox family of DNA binding transcription factors that has been shown to maintain pluripotency of embryonic stem cells. Nanog expression counteracts the differentiation-promoting signals induced by the extrinsic factors LIF, Stat3 and BMP. Once NANOG expression is downregulated, cell differentiation can proceed. Proteins which regulate NANOG expression include transcription factors Oct4, SOX2, FoxD3, and Tcf3 and tumor suppressor p53.

Product Specifications

Product Name Alternative

Nanog homeobox, hNanog, Homeobox protein NANOG, Homeobox transcription factor Nanog

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE, Denatured

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 80% by SDS-PAGE

Molecular Weight

34.6 kDa (305aa)

Additionnal Information

Nanog homeobox, Nanog, Homeobox transcription factor Nanog-delta 48, Homeobox transcription factor Nanog, Homeobox protein NANOG, hNanog, FLJ40451, FLJ12581, , , ATGP1142-10 µg, ATGP1142-20 µg, ATGP1142-50 µg, ATGP1142-100 µg, ATGP1142-250 µg, ATGP1142-500 µg, ATGP1142-1 mg, ATGP1142-010, ATGP1142-020, ATGP1142-050, ATGP1142-100, ATGP1142-250, ATGP1142-500, ATGP1142-01M

References & Citations

Clark A T., et al. (2004) Stem Cells. 22:169-179.; ; Chambers I., et al. (2003) Cell Res. 13:499-502.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_079141

Uniprot Accession Number

Q9H9S0

Species

Human

AA Sequence

MSVDPACPQS LPCFEASDCK ESSPMPVICG PEENYPSLQM SSAEMPHTET VSPLPSSMDL LIQDSPDSST SPKGKQPTSA EKSVAKKEDK VPVKKQKTRT VFSSTQLCVL NDRFQRQKYL SLQQMQELSN ILNLSYKQVK TWFQNQRMKS KRWQKNNWPK NSNGVTQKAS APTYPSLYSS YHQGCLVNPT GNLPMWSNQT WNNSTWSNQT QNIQSWSNHS WNTQTWCTQS WNNQAWNSPF YNCGEESLQS CMQFQPNSPA SDLEAALEAA GEGLNVIQQT TRYFSTPQTM DLFLNYSMNM QPEDV