Recombinant human HMGB2 protein

High mobility group protein B2, also known as HMGB2, is a member of the non-histone chromosomal high-mobility group protein family. The proteins of this family are chromatin-associated and ubiquitously distributed in the nucleus of higher eukaryotic cells. In vitro studies have demonstrated that this protein is able to efficiently bend DNA and form DNA circles. These studies suggest a role in facilitating cooperative interactions between cis-acting proteins by promoting DNA flexibility. This protein was also reported to be involved in the final ligation step in DNA end-joining processes of DNA double-strand breaks repair and V (D) J recombination. Recombinant human HMGB2 protein, fused to His-tag at N-terminus, was expressed in Baculovirus and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

High mobility group protein B2, HMG2

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

26.4 kDa (232aa)

Additionnal Information

HMGB2, High mobility group protein B2, HMG2, ATGP1141-10 µg, ATGP1141-20 µg, ATGP1141-50 µg, ATGP1141-100 µg, ATGP1141-250 µg, ATGP1141-500 µg, ATGP1141-1 mg, ATGP1141-10, ATGP1141-20, ATGP1141-50, ATGP1141-100, ATGP1141-250, ATGP1141-500, ATGP1141-1

References & Citations

Bustin M., et al. (1990) Biochem Biophys Acta. 1049:231-243.; ; Shirakawa H., et al. (1992) J Biol Chem. 267:6641-6635.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 30% glycerol, 0.1M NaCl

Scientific Category

Signal Transduction

NCBI Accession Number

NP_002120.1

Uniprot Accession Number

P26583

Species

Human

AA Sequence

MGKGDPN KPRGKMSSYA FFVQTCREEH KKKHPDSSVN FAEFSKKCSE RWKTMSAKEK SKFEDMAKSD KARYDREMKN YVPPKGDKKG KKKDPNAPKR PPSAFFLFCS EHRPKIKSEH PGLSIGDTAK KLGEMWSEQS AKDKQPYEQK AAKLKEKYEK DIAAYRAKGK SEAGKKGPGR PTGSKKKNEP EDEEEEEEEE DEDEEEEDED EE