Recombinant human HMGA1 protein
High mobility group protein HMG-I/HMG-Y, also known as HMGA1, is a member of the non-histone chromosomal high mobility group protein (HMG) family. HMGA1 consists of a highly conserved AT-hook DNA-binding domain that mediates binding to AT-rich sequences in the minor groove of chromosomal DNA. It functions as architectural chromatin-binding transcription factor altering the conformation of DNA by modulating nuclear protein-DNA complexes. It is involved in many cellular processes including growth regulation, viral induction of beta-IFN gene and regulation of inducible gene transcription. Recombinant human HMGA1 protein, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
High mobility group protein HMG-I/HMG-Y, HMG-R, HMGA1A, HMGIY
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by BCA assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
12.7 kDa (115aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
Additionnal Information
References & Citations
Chiappetta G., et al. (2004) Clin Cancer Res. 10 (22) :7637-44.; ; Massaad Massade L., et al. (2002) Biochemistry. 41:2760-2768.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 50% glycerol, 0.2M NaCl
Scientific Category
Signal Transduction
NCBI Accession Number
NP_665906.1
Uniprot Accession Number
P17096
Species
Human
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