Recombinant human ATF4 protein

Activating transcription factor 4, also known as ATF4, belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins and CREB-like proteins. ATF4 encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. Recombinant human ATF4 protein, fused to His-Calmodulin tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Activating transcription factor 4, CREB2, TXREB, CREB-2

Expression System

E.coli

Antigen Species

Human

Tag

His-Cam Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

56.6 kDa (510aa)

Additionnal Information

ATF4, ATGP1138-10 µg, ATGP1138-20 µg, ATGP1138-50 µg, ATGP1138-100 µg, ATGP1138-250 µg, ATGP1138-500 µg, ATGP1138-1 mg, ATGP1138-10, ATGP1138-20, ATGP1138-50, ATGP1138-100, ATGP1138-250, ATGP1138-500, ATGP1138-1

References & Citations

Lin Y S., et al. (1988) Proc Natl Acad Sci. 85:3396-4000.; ; Hoeffler JP., et al. (1988) Science. 242:1430-1433.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Scientific Category

Signal Transduction

NCBI Accession Number

NP_001666

Uniprot Accession Number

P18848

Species

Human

AA Sequence

M TEMSFLSSEV LVGDLMSPFD QSGLGAEESL GLLDDYLEVA KHFKPHGFSS DKAKAGSSEW LAVDGLVSPS NNSKEDAFSG TDWMLEKMDL KEFDLDALLG IDDLETMPDD LLTTLDDTCD LFAPLVQETN KQPPQTVNPI GHLPESLTKP DQVAPFTFLQ PLPLSPGVLS STPDHSFSLE LGSEVDITEG DRKPDYTAYV AMIPQCIKEE DTPSDNDSGI CMSPESYLGS PQHSPSTRGS PNRSLPSPGV LCGSARPKPY DPPGEKMVAA KVKGEKLDKK LKKMEQNKTA ATRYRQKKRA EQEALTGECK ELEKKNEALK ERADSLAKEI QYLKDLIEEV RKARGKKRVP