Recombinant human ACY3 protein

Aspartoacylase 3, also known as ACY3, is member of the Aspartoacylase subfamily. It plays an important role in deacetylating mercapturic acids in kidney proximal tubules. ACY3 localizes to the cytoplasm of S2 and S3 proximal tubules and to the apical domain of S1 proximal tubules. ACY3 protein is also expressed at low levels in stomach, testis, heart, brain, lung and liver, and may function as an HCV (Hepatitis C virus) core binding protein. Recombinant human ACY3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Aspartoacylase 3, ACY-3, HCBP1, Aminoacylase-3

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

37.6 kDa (342aa) confirmed by MALDI-TOF

Additionnal Information

ACY3, Aspartoacylase 3, ACY-3, HCBP1, Aminoacylase-3, ATGP1136-10 µg, ATGP1136-20 µg, ATGP1136-50 µg, ATGP1136-100 µg, ATGP1136-250 µg, ATGP1136-500 µg, ATGP1136-1 mg, ATGP1136-10, ATGP1136-20, ATGP1136-50, ATGP1136-100, ATGP1136-250, ATGP1136-500, ATGP1136-1

References & Citations

Chen Y R., et al. (2009) J Gastroenterol Hepatol. 24 (7) :1300-4.; ; Pushkin A., et al. (2004) . Am J Physiol Cell Physiol. 286 (4) :C848-56.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl

Scientific Category

Metabolism

NCBI Accession Number

NP_542389

Uniprot Accession Number

Q96HD9

Species

Human

AA Sequence

MCSLPVP REPLRRVAVT GGTHGNEMSG VYLARHWLHA PAELQRASFS AVPVLANPAA TSGCRRYVDH DLNRTFTSSF LNSRPTPDDP YEVTRARELN QLLGPKASGQ AFDFVLDLHN TTANMGTCLI AKSSHEVFAM HLCRHLQLQY PELSCQVFLY QRSGEESYNL DSVAKNGLGL ELGPQPQGVL RADIFSRMRT LVATVLDFIE LFNQGTAFPA FEMEAYRPVG VVDFPRTEAG HLAGTVHPQL QDRDFQPLQP GAPIFQMFSG EDLLYEGEST VYPVFINEAA YYEKGVAFVQ TEKFTFTVPA MPALTPAPSP AS