Recombinant human MPG protein
MPG (DNA-3-methyladenine glycosylase) belongs to the DNA glycosylase MPG family. This protein initiates base excision repair in DNA by removing a wide variety of alkylated, deaminated, and lipid peroxidation-induced purine adducts. Recombinant human MPG protein, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
DNA-3-methyladenine glycosylase, AAG, ADPG, anpg, APNG, CRA36.1, MDG, Mid1, PIG11, PIG16
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
33.9 kDa (306aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
Additionnal Information
References & Citations
Adhikari S., et al. (2009) DNA Repair (Amst) . 8 (10) :1201-6.; ; Allan, J.M., et al. (1998) Cancer Res. 58: 3965-3973.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 30% glycerol, 200 mM NaCl, 1mM EDTA
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_002425.2
Uniprot Accession Number
P29372
Species
Human
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